以代谢物组学为参照介绍血液指纹图谱

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<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">以代谢物组学为参照介绍血液指纹图谱中医药信息学研究方法</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 54pt; mso-char-indent-count: 4.5"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">赵宏杰（吉林市中医院</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">吉林</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">吉林</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman"> 132011</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">         </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">雷钧涛</span><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">  </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">（吉林医药学院</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">吉林</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">吉林</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman"> 132011</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">摘</span><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">  </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">要：血液指纹图谱中医药信息学研究方法是一种新的中医药现代化研究方法，为方便大家了解和认识这一方法，特以代谢物组学为参照介绍了它的一些特点。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">中国图书资料分类号：</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">R2-03</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">　　</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">文献标识码：</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">A<p></p></font></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">主题词</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">: </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">代谢物组学</span><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">  </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">血液指纹图谱</span><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">  </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">中医药信息学</span><span lang="EN-US" style="FONT-SIZE: 12pt"><span style="mso-spacerun: yes"><font face="Times New Roman">  </font></span></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">藏象信息模型</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="FONT-SIZE: 12pt"><p><font face="Times New Roman"></font></p></span></p><p><font face="Times New Roman"></font></p><p></p><p><font face="Times New Roman"></font></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">近年来，笔者结合系统生物学、中医药信息学和生物信息学等学科的最新研究成果，提出了血液指纹图谱藏象信息模型这一中医药信息学研究新方法。为方便大家了解和认识这一方法，下面以代谢物组学为参照介绍它的一些特点。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">[1]-[5]<p></p></font></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="FONT-SIZE: 12pt"><p><font face="Times New Roman"></font></p></span></p><p><font face="Times New Roman"></font></p><p></p><p><font face="Times New Roman"></font></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">1.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">代谢物组学</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">代谢物组学是以代谢物分析的整体方法来研究功能蛋白如何产生能量和处理体内物质，评价细胞和体液的内源性和外源性代谢物浓度与功能关系的学科。是系统生物学的重要组成部分。通常采用绘图技术，现代分析测定方法</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">(NMR,HPLC,MS)</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">，以及应用计算机技术和统计学方法，以高通量实验和大规模计算为特征，完成细胞或者生物样品所有代谢物的“指纹图谱”。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">其核心思想是它能够通过整体代谢物图谱，直接认识生物体的生理和生化状态</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">代谢物组学通过研究</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">&quot;</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">代谢指纹图谱</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">&quot;</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">的变化，能更直接地反映体内生物化学过程和状态的变化。其研究对象主要是生物体液，如尿液、血液，主要研究手段是核磁共振。代谢物组学在药物模型识别和确证、作用机制研究和安全性评价等方面具有重要的理论意义和应用价值。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman"> [6]-[7]<p></p></font></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">2.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">血液指纹图谱中医药信息学研究方法</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">在日、年等完整的生命节律时段内，连续提取血液并制作血液指纹图谱，根据中医子午流注和藏气法时等中医时间医学理论，构建动态的——有时间变量的中医学的人体生命信息模型，血液指纹图谱藏象信息模型。实现中医学的理论表达，并在此基础上以信息技术实现中医学的研究与实际应用。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">[1]-[5]<p></p></font></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">3.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">以代谢物组学为参照介绍血液指纹图谱中医药信息学研究方法</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">3.1</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">指纹图谱方法是一种信息学方法，代谢物组学研究表明，指纹图谱方法可以直接认识生物体的生理和生化状态和随时空变化的情况。不仅关心代谢物质的整体也关注其动态变化规律。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">3.2</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">血液指纹图谱能比代谢指纹图谱，更完整的认识生物体的生理和生化状态</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">。代谢物组指生物体内的所有小分子代谢物，是生物体的特定部分在特定环境中，经基因组表达和新陈代谢产生的中介物及终产物。血液所包含的成分比这还要多，血液大致相当于系统整体代谢物组。所以，血液指纹图谱能比代谢指纹图谱，更完整的认识生物体的生理和生化状态</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">.</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">3.3</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">中医通过子午流注和藏气法时等中医时间医学理论，对生物体的生理和生化状态即功能单位进行划分，系统生物学以信息建模与系统整合为目标，血液指纹图谱藏象信息模型实现了这一目标，而且遵循了中医对系统</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">“由上而下”划分的原则，进行的信息建模。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">3.4</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">藏象生理血液指纹图谱即人体正常的生理和生化状态，藏象病理血液指纹图谱即人体异常的生理和生化状态，也就是证的血液指纹图谱。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">1992</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">年程士德教授在他编写的《内经理论体系纲要》一书中说：“藏象学说的实质就是关于人体形态结构、生理功能、物质代谢、病理变化的高度概括，是一个综合性的概念。”</span><span style="FONT-SIZE: 12pt"><font face="Times New Roman"> </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">中医药学从认识和掌握人体功能状态的变化规律入手，初步揭示了人体生命活动的变化规律。证候既然是中医对疾病过程中人体功能状态的概括和总结，因此，用生理学方法探讨中医证候是证候研究的前提。。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">　　中医学的证候的观点，即对人体状态加以认识、分类和调控的观点，是目前现代医学对人体状态分类所没有的，通过中医对人体所处证候的分类，用现代科技手段通过对生理信息的处理，从而认识人体的状态特征，是中医学证候现代研究的基础性和前提工作。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman"><span style="mso-spacerun: yes"> </span><span style="mso-spacerun: yes">   </span></font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">证是人体在疾病过程中某一阶段的病理概括，是机体对病因的整体反应状态，它反映了藏象、经络、气血津液等功能结构的异常变化。不同的证候标示着机体不同层面的整体功能异常，“中医把认识疾病的焦点放在了整体功能上，用各种辨证方法把病人身上出现的整体功能异常辨别为各种有特异临床表现的证，每一种证实际上都是有特征内容和表现的整体功能异常态”，可见，证的本质应是整体功能的，而不是解剖形态的。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">[1]-[]<p></p></font></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">证本质研究又是证型规范化的前提与基础，是中、西医两种医学体系深层次沟通的必由途径。所以深入地探索中医“证”的诊断或评价指标、尝试对其进行定性定量的表达研究，是中医证型规范化及建立中医疗效评价指标体系的重要指标，具有实际的临床应用价值。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">4. </font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">血液指纹图谱突破了制约代谢物组学中医研究的瓶颈</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">目前，代谢物组学通常采取核磁共振（</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">NMR</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）、质谱（</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">S</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）、高效液相色谱（</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">HPLC</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）为代谢物组“绘制图谱”。代谢组学之父、英国伦敦帝国理工学院</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">Jeremy Nicholson</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">教授认为，人体应该作为一个完整的系统来研究，应用代谢组学和全面性系统策略来理解疾病过程，与中医的整体观念和辨证论治思维方式不谋而合。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-INDENT: 24pt; mso-char-indent-count: 2.0"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">中医界非常多的学者也赞同这个观点，但是，（</span><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman">NMR</font></span><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">）这样的昂贵设备限制了代谢物组学中医研究的开展。而血液指纹图谱藏象信息模型的研究，主张以红外和拉曼光谱制作血液指纹图谱，这样，既可以保证指纹图谱的测定简单、可行、有效，又保证了指纹图谱所必须具备的特异性、重现性和再现性。而且大大的降低了血液指纹图谱研究的成本，以红外和拉曼光谱制作代谢指纹图谱，也将大大降低代谢物组学中医研究的成本，推动这一新兴学科快速发展。</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span style="FONT-SIZE: 12pt; FONT-FAMILY: 宋体; mso-hansi-font-family: &quot;Times New Roman&quot;; mso-ascii-font-family: &quot;Times New Roman&quot;">参考文献：</span><span lang="EN-US" style="FONT-SIZE: 12pt"><p></p></span></p><p></p><p></p><p></p><p></p><span lang="EN-US" style="FONT-SIZE: 12pt"><font face="Times New Roman"><p></p></font></span><p></p><p></p><p></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt"><span lang="EN-US" style="FONT-SIZE: 12pt"><p><font face="Times New Roman"></font></p></span></p><p><font face="Times New Roman"></font></p><p></p><p><font face="Times New Roman"></font></p><p></p>

老包

<p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman" size="2"><strong><font color="#ff0000">中药指纹图</font></strong>关键技术＂<font color="#ff0000"><strong>气相色谱</strong></font>＂一例：</font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font face="Times New Roman" size="2"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman" size="2"></font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman" size="">50%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">丙草胺水乳剂是最新研制成功的环保型水稻田除草剂，可有效防除水稻田千金子、</span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体"><font size="2">稗草、异型莎草、牛毛毡、泽泻、萤蔺、鸭舌草等多种杂草。杀草谱广、防效稳定。本文采</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体"><font size="2">用气相色谱内标法对丙草胺进行了定量分析，其方法准确、简便、灵敏度高、重复性好，可</font></span><font size="2"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">作为</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">50%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">丙草胺水乳剂生产控制分析方法。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ºÚÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ºÚÌå"><font face="Times New Roman">1 </font></span><span style="FONT-FAMILY: 黑体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 黑体">实验部分<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ¿¬Ìå_GB2312; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ¿¬Ìå_GB2312"><font face="Times New Roman">1.1 </font></span><span style="FONT-FAMILY: 楷体_GB2312; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 楷体_GB2312">仪器与试剂<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.1.1</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">仪器 气相色谱仪：日本岛津</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">GC<chmetcnv w:st="on" unitname="C" sourcevalue="14" hasspace="False" negative="True" numbertype="1" tcsc="0">-14C</chmetcnv></font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；氢火焰离子化检测器；色谱数据处理机：<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">C-R<chmetcnv w:st="on" unitname="a" sourcevalue="8" hasspace="False" negative="False" numbertype="1" tcsc="0">8A</chmetcnv></font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；进样器：</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">10</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">μ</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">L</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.1.2</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">试剂 三氯甲烷：分析纯；内标物：乙草胺，不含干扰分析的杂质；内标溶液：称<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">取</span><font face="Times New Roman"><chmetcnv w:st="on" unitname="g" sourcevalue=".5" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">0.5g</span></chmetcnv><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">乙草胺于</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">100mL </font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">容量瓶中用三氯甲烷稀释，定容；丙草胺标样：已知含量，≥</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">98.0%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ¿¬Ìå_GB2312; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ¿¬Ìå_GB2312"><font face="Times New Roman">1.2 </font></span><span style="FONT-FAMILY: 楷体_GB2312; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 楷体_GB2312">色谱条件色谱柱</span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">： </span><chmetcnv w:st="on" unitname="m" sourcevalue="2" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">2.0m</font></span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">×</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">3.2 </font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">㎜不锈钢填充柱</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">,</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">内装</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">5%SE-30/Chromosorb W AW-DMCS(150</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">～</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">180um)</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">填充物；载气</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">:40mL/min</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">（氮气）；氢气：</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">40mL/min</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；空气：</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">400mL/min</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；进样口温度：</span><chmetcnv w:st="on" unitname="℃" sourcevalue="250" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">250</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；检测器温度：</span><chmetcnv w:st="on" unitname="℃" sourcevalue="250" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">250</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；柱温：</span><chmetcnv w:st="on" unitname="℃" sourcevalue="215" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">215</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">℃</span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">；进样量：</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">1</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">μ</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">L</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ¿¬Ìå_GB2312; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ¿¬Ìå_GB2312"><font face="Times New Roman">1.3 </font></span><span style="FONT-FAMILY: 楷体_GB2312; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 楷体_GB2312">实验方法<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.3.1</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">内标溶液的配制 称取</span><font face="Times New Roman"><chmetcnv w:st="on" unitname="g" sourcevalue=".5" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">0.5g</span></chmetcnv><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">乙草胺于</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">100mL </font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">容量瓶中，用三氯甲烷溶解并定容，</span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体"><font size="2">充分摇匀后备用。<span lang="EN-US"><p></p></span></font></span></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.3.2</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">标准溶液的配制 称取丙草胺标样</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">100mg</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">（精确至</span><chmetcnv w:st="on" unitname="g" sourcevalue=".0002" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">0.0002g</font></span></chmetcnv><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">），于</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">15mL </font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">三角瓶中，准</span></font><font size="2"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">确加入内标液</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">5mL</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">，补加</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">5mL</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">三氯甲烷溶解，摇匀。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.3.3</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">试样溶液的配制 称取</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">50%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">丙草胺水乳剂</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">200mg(</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">精确至</span><font face="Times New Roman"><chmetcnv w:st="on" unitname="g" sourcevalue=".0002" hasspace="False" negative="False" numbertype="1" tcsc="0"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">0.0002g</span></chmetcnv><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">)</span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">，于</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">15mL</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">三角瓶中，</span></font><font size="2"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">准确加入内标液</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">5mL</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">，加</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">5mL</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">三氯甲烷溶解，摇匀。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><font face="Times New Roman"><chsdate w:st="on" year="1899" month="12" day="30" islunardate="False" isrocdate="False"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå">1.3.4</span></chsdate><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"> </span></font><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">测定与计算 待仪器按要求条件稳定后，连续进数针标样溶液，直至相邻两针丙草</span></font><font size="2"><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">胺相对响应值变化小于</span><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">1.5%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">时，按标样溶液、试样溶液、试样溶液、标样溶液顺序进样。<span lang="EN-US"><p></p></span></span></font></p><p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; TEXT-ALIGN: left; mso-layout-grid-align: none" align="left"><font size="2"><span lang="EN-US" style="FONT-FAMILY: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><font face="Times New Roman">50%</font></span><span style="FONT-FAMILY: 宋体; mso-hansi-font-family: ËÎÌå; mso-bidi-font-size: 10.5pt; mso-font-kerning: 0pt; mso-bidi-font-family: 宋体">丙草胺水乳剂气相色谱图见图。</span></font><span lang="EN-US" style="FONT-SIZE: 10pt; FONT-FAMILY: ËÎÌå; mso-font-kerning: 0pt; mso-bidi-font-family: ËÎÌå"><p></p></span></p>